Extended Data Fig. 3: Additional analysis of CITE-Seq of splenic pDCs.
From: Tonic type I interferon signaling optimizes the antiviral function of plasmacytoid dendritic cells
Sorted pDCs from spleens of three individual naive mice were hashtagged and analyzed by CITE-Seq for single-cell transcriptome and phenotype (antibody-derived tags, ADT). (a) UMAP plot and clustering of the dataset prior to the tDC cluster removal (clusters are numbered and colored randomly). (b-c) Violin plots showing transcript levels of key pDC (b) and tDC (c) identity genes. (d) Heatmap of top 10 marker gene expression based on mRNA transcripts or ADT reads for the clusters in panel A. (e) Heatmap of ADT for markers expressed in pDCs across the three Ct clusters defined in Fig. 2b. (f) RNA velocity analysis. Shown are velocity vectors projected onto the KnetL and UMAP plots from Fig. 2b, with the three Ct clusters indicated.
