Extended Data Fig. 4: Verification of transcriptional heterogeneity in murine splenic pDCs.
From: Tonic type I interferon signaling optimizes the antiviral function of plasmacytoid dendritic cells
A previously reported scRNA-seq dataset of splenic pDCs from naive mice (GSE114313) was reanalyzed to assess steady-state heterogeneity. (a) UMAP plot, with clusters from the KNetL clustering in the next panel indicated by colors. (b) KNetL plot and clustering. (c) Violin plots showing transcript levels of key pDC identity genes by KNetL clusters. (d) Heatmap of marker gene expression for KNetL clusters. (e) Feature plots of key marker gene transcripts on the KNetL plot from panel B; cluster with prominent ISG expression (cluster 2) is highlighted. (f) The expression of MHC cl II transcript shown on the feature plot (with the MHC cl. II-low cluster 5 highlighted) and violin plot.
