Extended Data Fig. 7: SK56 inhibits cell death in human blood under pyroptosis condition.
From: Delaying pyroptosis with an AI-screened gasdermin D pore blocker mitigates inflammatory response
Flow cytometry assay show changes in cell populations in peripheral blood samples from healthy volunteers (n = 7) and sepsis patients (n = 6; within 72 hours of sepsis confirmation). Blood from healthy volunteers was treated at 37 °C for 4 hours with 1 μg/ml LPS, 10 μM nigericin to induce pyroptosis, and DSF (disulfiram, 30 μM added 30 min before inducing pyroptosis). All graphs present mean ± s.d. Statistical significance was assessed using two-way ANOVA (NS, P > 0.05, no significance; *P < 0.05, **P < 0.01).
