Extended Data Fig. 7: Validation of LyP subcluster annotation and differential lineage bias. | Nature Immunology

Extended Data Fig. 7: Validation of LyP subcluster annotation and differential lineage bias.

From: Single-cell multiomic atlas of healthy pediatric bone marrow reveals age-dependent differences in lineage differentiation driven by stromal signaling

Extended Data Fig. 7

a) CITE-seq based violin plots showing the relative expression levels of DSB-normalized ADT counts for shown surface markers in LMPP, LyP-S, LyP-B and pro B cells (cycling), as identified in Fig. 1b and Fig. 3a. b) Violin plots showing the relative expression of the top10 marker genes for lymphoid progenitor cells from the DISCO single-cell reference atlas19. c) Tile plot showing the percentage of cells for each LyP subcluster mapping to each of the cell clusters in the DISCO single-cell reference atlas19. d) CITE-seq based diffusion map, showing the differentiation trajectory from HSC to B cell and myeloid lineages, visualizing all the intermediary cell types. e) As in d) highlighting the Lyp-B and LyP-S subclusters. Abbreviations: LyP, lymphoid progenitor; pDC, plasmacytoid dendritic cell; GMP, granulocyte-monocyte progenitor; CDP, common dendritic cell progenitor; pre-pDC, precursor plamacytoid DC; CMP, common myeloid progenitor; HSC, hematopoietic stem cell; LMPP, lympho-myeloid primed progenitor.

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