Extended Data Fig. 6: Vaccine-induced protection in the two subgroups of immunized macaques challenged with SHIV AD8.

Subgroup 1 received 5 mRNA and 5 protein immunizations; subgroup 2 received 8 mRNA and 2 protein immunizations. a. Kaplan-Meier analysis of virus-free survival in the course of 13 weekly intrarectal inoculation of 10 TCID₅₀ of SHIV AD8 (red arrows) in the two subgroups of immunized macaques. Infection was evaluated by the appearance of plasma viremia on two subsequent tests using a sensitive real-time PCR method. Significance was calculated by the Wilcoxon exact test. b. Statistical analysis of hazard ratio and per-exposure risk in the two subgroups of immunized macaques, as assessed by 1 minus the hazard ratio, estimated from a Cox proportional hazards regression via the exact partial likelihood with group (vaccinated versus control) as the regressor. c. Mean levels of viremia (± SD) in macaques in the two vaccine subgroups synchronized by peak of viremia. Levels of viremia were evaluated by quantitative real-time PCR. The courses of viremia in different animals were aligned by setting the peak of viremia at day 21 for each animal.