Extended Data Fig. 8: Polyclonal epitope mapping of trimer-binding antibodies in a representative protected animal by negative-staining electron microscopy (NSEM). | Nature Medicine

Extended Data Fig. 8: Polyclonal epitope mapping of trimer-binding antibodies in a representative protected animal by negative-staining electron microscopy (NSEM).

From: A multiclade env–gag VLP mRNA vaccine elicits tier-2 HIV-1-neutralizing antibodies and reduces the risk of heterologous SHIV infection in macaques

Extended Data Fig. 8

The analysis was performed using purified Fab fragments isolated from serum collected from macaque V3 (subgroup 1) at week 58. The arrow heads point to the trimer-bound Fab fragments. Visualization of antibodies binding to: a. A region compatible with the CD4-BS deep in the inter-protomer groove; b. A region in the inter-protomer groove more distal from the trimer axis than the CD4-BS; c. A region(s) at the trimer apex targeted by 2 Fabs simultaneously; d. A region at the trimer apex compatible with the V2-glycan supersite targeted by a single Fab; e. A region at the trimer base, presumably constituted by neo-epitopes produced by gp41 truncation.

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