Fig. 3: Demonstration of SIMFLUX on cellular tubulin with DNA-PAINT and (d)STORM.
From: Localization microscopy at doubled precision with patterned illumination
a, Full 26-μm wide FOV SIMFLUX image of tubulin sample imaged with DNA-PAINT. Three independent imaging experiments were done with similar outcome. Scale bar, 5 μm. b–d, Zoom-in on SMLM and SIMFLUX images of boxes in a; all reconstructions are based on the same underlying data. Scale bar in b (for b and c), 1 μm; in d, 2.5 μm. e, Cross-section histogram of the tubulin segment in d with bimodal Gaussian fit. f, Localization error Δloc and CRLB (mean and s.d.) determined from repeated localizations of long molecular on-events in the dataset of a. g, FRC curves for dataset of a with resolution values R. h, Full 26-μm wide FOV SIMFLUX image of tubulin sample imaged with (d)STORM. Four independent imaging experiments were done with similar outcome. Scale bar, 5 μm. i–k, Zoom-in on SMLM and SIMFLUX images of boxes in h, both reconstructions are based on the same underlying data. Scale bar in i (for i and j), 1 μm; in k, 2.5 μm. l, Cross-section histogram of the tubulin segment in k with bimodal Gaussian fit. m, Localization error Δloc and CRLB (mean and s.d.) determined from repeated localizations of long molecular on-events in the dataset of h. n, FRC curves for dataset of h with resolution values R. Number of localizations per data point in f and m are given in the Supplementary Table.
