Extended Data Fig. 9: Effect of mismatched base position on xABE activity and Sanger sequencing results of editing human endogenous transcripts by xABE.
From: Programmable RNA editing with compact CRISPR–Cas13 systems from uncultivated microbes
a, Mismatch positions in different crRNAs targeting the mCherry amber mutation. b, Effect of mismatched base position with 50-nt spacer on A-to-I editing efficiency by both full and mini xABE editors. All values are presented as mean ± s.e.m (n = 3). c, Sanger sequencing results showing representative A-to-I conversion on endogenous transcripts by full and mini xABE editors. Red triangles indicate mutation sites.
