Extended Data Fig. 8: Validation of in situ PSF modelling for imaging a thick sample. | Nature Methods

Extended Data Fig. 8: Validation of in situ PSF modelling for imaging a thick sample.

From: Universal inverse modeling of point spread functions for SMLM localization and microscope characterization

Extended Data Fig. 8

Imaging of Nup96-SNAP-AF647 in U2OS cells was performed using an oil immersion objective lens (NA = 1.5) and imaging buffer (n = 1.406, Methods) on a single-channel SMLM system equipped with a deformable mirror (DM). (a) A sandwiched sample of U2OS cells was prepared for an imaging depth of 25 µm. (b) Top view of the reconstructed Nup96 using the in situ PSF model. (c) Enlarged XY view of the selected region in (b). (d) XZ view of the selected region in (c) reconstructed from bead and in situ PSF models, respectively. Scale bars, 2 µm (b), 1 µm (c), and 0.5 µm (d).

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