Extended Data Fig. 9: Application of uiPSF for aberration correction using a deformable mirror.
Imaging of Nup96-SNAP-AF647 in U2OS cells was performed using an oil immersion objective lens (NA = 1.5) and imaging buffer (n = 1.406, Methods) on a single-channel SMLM system equipped with a deformable mirror (DM). (a) Top view of the reconstructed Nup96 using the in situ PSF model with and without uiPSF guided aberration correction by AO. (b) Zernike aberrations calculated from single molecule data using uiPSF with and without AO correction. (c, d) XZ view of the selected region in (a) for samples with and without AO correction. After uiPSF guided aberration correction, the reconstructed image quality improved notably as demonstrated by the resolved double ring structure. Scale bars, 1 µm (a), 500 nm (c-d).
