Extended Data Fig. 6: Comparison between FICTURE and JSTA on simulated data.

(a) We simulated the data using the simulation code provided in JSTA publication. We simulated a 200 µm x 200 µm area with 2 pixels per µm. We simulated 8 cell types from the single cell reference data as used in JSTA publication. (b) Runtime (left, wall time) and maximum memory usage (right) of the two methods, presented as mean +/- SD. We used 4 CPUs for both methods and repeated the simulation 20 times. (c) Visualization of the pixel level cell type assignment errors of FICTURE (left) and JSTA (middle). Pixels classified correctly are left as white while the errors are visualized with colors corresponding to true cell types. (d) Visualization of the pixel level cell (nuclei) assignment errors of FICTURE (left) and JSTA (middle). The mean +/- SD of the cell type and cell assignment error rate over n = 20 simulations are shown on the right side of (C) and (D), respectively.