Extended Data Fig. 1: Extended field-of-views of hybrid DNA constructs immobilized on graphene. | Nature Methods

Extended Data Fig. 1: Extended field-of-views of hybrid DNA constructs immobilized on graphene.

From: Single-molecule dynamic structural biology with vertically arranged DNA on a fluorescence microscope

Extended Data Fig. 1

Systems containing dsDNA segments of 36 bp, 45 bp, 51 bp and 66 bp length are depicted. For the 36 bp, 51 bp and 66 bp cases the white dotted boxes mark the areas shown in Fig. 1b. The histograms of non-deconvoluted fluorescence lifetime of each detected spot show that the fluorescence lifetimes are homogeneous for large areas. A shift to larger fluorescence lifetimes is observed when the length of the dsDNA segment is increased.

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