Extended Data Fig. 3: Comparison of SABER and pSABER on a slide-scanning microscope. | Nature Methods

Extended Data Fig. 3: Comparison of SABER and pSABER on a slide-scanning microscope.

From: Efficient and highly amplified imaging of nucleic acid targets in cellular and histopathological samples with pSABER

Extended Data Fig. 3

a, SABER (left) and pSABER (right) visualization of mouse major satellite DNA imaged using a 10x air objective. b, SABER (left) and pSABER (right) visualization of mouse major satellite DNA imaged using a 20x air objective. c, SABER (left) and pSABER (right) visualization of mouse major satellite DNA imaged using a 10x air objective. Each image is presented at the indicated high (top) and low (bottom) contrast settings to allow for visual comparisons between signals occupying different portions of the 16-bit dynamic range (0–65535). Images are maximum projections in Z. Scale bars, 20 µm (insets) or 100 µm (fields of view). Each experiment was repeated ≥3 times and yielded similar results.

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