Extended Data Fig. 4: Efficiency and fidelity of Exchange-pSABER.

a, Schematic of Exchange-pSABER in which exchange occurred after hybridization with an HRP-oligo but before localized label deposition to assess exchange efficiency. b, Representative images of DNA pSABER targeting mouse minor satellite in EY.T4 mouse embryonic fibroblasts using three different combinations of HRP-oligo and fluorescent tyramide. The mock condition proceeded through pSABER as normal whereas the exchange condition developed after displacing the HRP-oligos. Images are displayed at the indicated high (top) and low (bottom) contrast settings to illustrate exchange efficiency. Scale bars, 10 µm. Each experiment was repeated ≥3 times and yielded similar results.