Fig. 1: A spatial mechano-transcriptomics framework applied to a E8.5 mouse embryo seqFISH dataset.
From: A computational pipeline for spatial mechano-transcriptomics
a, An overview of the spatial mechano-transcriptomics pipeline, showing immunostained membrane images and single-cell RNA-sequencing references as inputs, followed by deep learning segmentation, tension and pressure inference, gene expression imputation and final integrated mechanical-transcriptomic output maps. b, A schematic (top left) and images of the two different embryo sagittal sections considered in this study with close-ups of the three different brain regions studied in more details thereafter. Dataset 1: FMH and NC regions of embryo 1 brain; dataset 2: CM and FHM regions of embryo 2 brain; and dataset 3: MHB region of embryo 2 brain. Panels adapted from: a (bottom left), ref. 24 under a Creative Commons license CC BY 4.0; b (top left and middle), ref. 24 under a Creative Commons license CC BY 4.0.
