Extended Data Fig. 3: Investigating DeepSPT’s robustness to simulation parameters for the heterogeneous diffusing test set in 2D.

Investigation of model generalizability and limitations for key simulation parameters for traces containing all 4 diffusional types at random (see Methods for test set elaboration): a, Varying ranges of diffusion coefficients (D) for simulated trajectories (varying D is equivalent to varying temporal resolution for observation of a diffusing particle due to scale invariance of diffusion). b, step length to localization error ratio, i.e ratio of contribution to displacements from actual diffusion and localization error respectively where a value of 1 signifies an equal contribution and >1 signifies actual diffusion being larger. c, Duration of track. Median accuracy and mean accuracy are track-level metrics providing descriptive statistics for the distribution track-level accuracies for each test set track (N=20000). Flattened accuracy measures the accuracy for all frame-level predictions inside each trajectory pooled together.