Extended Data Fig. 3: BD626HTL can offer 25 z-frames for 3D reconstruction, enabling the construction of the distribution of the cytoskeleton (Cep41) throughout the entire cell. | Nature Methods

Extended Data Fig. 3: BD626HTL can offer 25 z-frames for 3D reconstruction, enabling the construction of the distribution of the cytoskeleton (Cep41) throughout the entire cell.

From: A palette of bridged bicycle-strengthened fluorophores

Extended Data Fig. 3

Orthogonal views of 3D-STED images of fixed HeLa cells expressing Cpe41-HT7 labeled with BD626HTL (500 nM, 30 min, 37 °C, one wash). An area of 52.5 × 51.0 μm (x–y) was recorded in 65 nm z-stacks over 25 times; scale bar= 1 μm.

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