Extended Data Fig. 3: Comparison between SLIM reconstruction and ground truth.
From: Kilohertz volumetric imaging of in vivo dynamics using squeezed light field microscopy
High-resolution ground truth images are acquired with a reference camera (sharing same objective with SLIM) under either widefield or light-sheet illumination. Sample is axially scanned with a motorized stage to acquire a z stack. SLIM and ground truth image are acquired sequentially on the same sample. a. Tilted mouse kidney slice (FluoCells™ Prepared Slide from ThermoFisher) b. Live mouse hippocampus (CA1, excitatory pyramidal neurons). Green arrows denote representative neuron candidates. Yellow arrows mark the overlaps between sub-aperture views (fabrication tolerance of prisms and holder). When sample exhibits a strong background, this overlap could induce reconstruction artefacts. Blue arrows indicate the MIP artifacts due to the bright boundary of circular FOV. c. Embryonic zebrafish vasculature (endothelial cells, Tg(flk:mCherry)). Scale bar, 100 μm.
