Fig. 4: Correction of aberrations in live SUM159-AP2 cells expressing σ2-eGFP. | Nature Methods

Fig. 4: Correction of aberrations in live SUM159-AP2 cells expressing σ2-eGFP.

From: Fourier-based three-dimensional multistage transformer for aberration correction in multicellular specimens

Fig. 4

a, 3D volume SUM159-AP2 cells represented as xyMIPs and yzMIPs covering a 15.7 × 55.6 × 25.6 μm3 FOV after applying a 2.9λ P–V aberration to the DM. This aberration combines horizontal coma \({Z}_{3}^{1}\) and oblique trefoil \({Z}_{3}^{3}\). b, xyMIPs and yzMIPs of a similar FOV with 3.1λ P–V aberration composed of horizontal coma (\({Z}_{3}^{1}\)) and primary spherical (\({Z}_{4}^{\,0}\)). In both cases, near diffraction-limited performance was recovered after two iterations. Insets: FFTs and corresponding wavefronts for each iteration.

Back to article page