Fig. 6: Correcting aberrations postacquisition using spatially varying deconvolution in a zebrafish embryo. | Nature Methods

Fig. 6: Correcting aberrations postacquisition using spatially varying deconvolution in a zebrafish embryo.

From: Fourier-based three-dimensional multistage transformer for aberration correction in multicellular specimens

Fig. 6

a, Isoplanatic patch map determined by AOViFT for 204 tiles (6.3 × 6.3 × 12.8 μm3 each), spanning 37 × 211 × 12.8 μm3 FOV in a live, gene-edited zebrafish embryo expressing endogenous AP2-mNeonGreen. The yellow box marks areas with insufficient spatial features to accurately determine aberrations; an ideal PSF was used for OMW deconvolution in these regions. b, xyMIP of the AP2 signal without AO. c, xyMIP of each tile after deconvolution with spatially varying PSFs predicted by AOViFT. d,e, Raw (d) and deconvolved (e) xyMIPs of the mitochondria channel for the same region. f, Enlarged view of the wavefronts within the black dashed box in a. g,h, Zoomed-in views of AP2 (g) and mitochondria (h) structures from b–e, comparing No AO to OMW deconvolution using either an ideal PSF or spatially varying tile-specific aberrated PSFs predicted by AOViFT.

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