Extended Data Fig. 9: C. elegans tracking.
a) Z-slices of 3D confocal data for C. elegans embryogenesis (training data). b) Using retrained neural networks, we obtain well-calibrated link-likelihood predictions (top panel). Almost all links in the tracking solution that minimize the global energy (blue) have very high marginalized likelihoods (bottom panel). In this data set, only few links in the graph are not in the tracking solution (grey) as the nuclei are less closely packed then in intestinal organoid data. c) Lineage trees with associated error rates show that only few potential errors (41 links with an error probability above 1%) are present after automated tracking. d) Lineage trees after manual correction of potential errors. The lineages map exactly on the known C. elegans AB, e, MS, c, d and P4 sub-lineages, strongly suggesting that no errors remain. e) Tracking statistics show a very low error rate and that almost no error in linking. Most errors arise from undetected nuclei (under-segmentation), deep in the imaging volume.
