Fig. 3: Interaction of methylation maintenance and nucleosome positioning. | Nature Methods

Fig. 3: Interaction of methylation maintenance and nucleosome positioning.

From: Single-cell multi-omic detection of DNA methylation and histone modifications reconstructs the dynamics of epigenomic maintenance

Fig. 3

a, Overview of single-molecule analysis. Average methylation values are computed with respect to the start of the read, which is the MNase cut site within linker DNA. Figure created with BioRender.com. b, Heatmap of normalized methylation across the cell cycle at different distances from MNase cut site. c, Line charts of average methylation change across the cell cycle for three representative cut site distances. While distances of 90 and 270 bp represent nucleosome-covered regions, 180 bp consists of the linker DNA region. d, Relative change of CpG methylation (y axis, percent point from total) as a function of distance from the MNase cut, which are colored and split by chromatin domain. The line indicates LOESS smoothened representation of underlying data points and error band indicates the s.e. of the fit.

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