Extended Data Fig. 3: Comparison of CpG methylation data.
a. Bar plot showing genome-wide single-cell CpG coverage in the K562 scEpi2-seq data set. b. Correlation between pseudobulk DNA methylation data extracted from the TAPS portion of K562 scEpi2-seq data and ENCODE WGBS data for different cell lines. Correlation is calculated for non-overlapping 10 kb bins with a coverage of at least 50 reads per bin. c. Correlation between the TAPS portion of K562 scEpi2-seq data and ENCODE WGBS at single-CpG resolution. X-axis represents different cutoffs (0 = no filtering, 1 = more than 1 read etc.). For comparison, WGBS data were downsampled to read depths as observed in our scEpi2-seq data. d. scEpi2-seq traces for different histone modifications (left) and corresponding CpG methylation (right) for K562 cells. The line plots contain comparisons between pseudobulk values (maximum normalized values for histone modifications and average CpG methylation for DNA methylation) from scEpi2-seq and corresponding measurements for bulk ENCODE data (ChIP for histone modifications and WGBS for CpG methylation).
