Supplementary Figure 5: Membrane properties of neurons expressing somatic vs. untargeted molecules

Cultured hippocampal neurons expressing CoChR-GFP (n = 10 cells from 3 cultures), KA2(1-150)-GFP (n = 10 cells from 3 cultures), and soCoChR-GFP (n = 10 cells from 3 cultures) were patched 14 days following AAV transduction. (a) Resting potential. We performed a Kruskal-Wallis Test (not significant, P = 0.7549, chi-square = 0.5624). (b) Holding current. Kruskal-Wallis Test, not significant, P = 0.9875, chi-square = 0.0252. (c) Membrane capacitance. Kruskal-Wallis Test, not significant, P = 0.9817, chi-square = 0.0369. (d) Membrane resistance. Kruskal-Wallis Test, not significant, P = 0.9205, chi-square = 0.1656. For all panels, red line denotes the median. Top and bottom edges of the box indicate the 75th and 25th percentiles, respectively. Top and bottom whiskers indicate the highest and lowest values.