Supplementary Figure 7: 2P action spectrum and normalized excitation cross section of CoChR

(a) Normalized peak current of CoChR as a function of wavelength, measured in cultured CHO cells. Photostimulation was performed using a 2P Gaussian spot (FWHM = 38 µm) at different wavelengths, at constant photon flux (2.4 ± 0.3 10²⁶ photons/s·m²) over the investigated wavelength range. Plotted data are mean ± s.e.m., with raw data in open circles, n = 5 cells. (b) Normalized 2P excitation cross section, extracted from the photocurrent kinetics (see Methods). Plotted data are mean ± s.e.m., with raw data in open circles, n = 5 cells.