Supplementary Figure 9: Fluorescence plots along neurites, for cells expressing CoChR-GFP or soCoChR-GFP in slices

(a-b) Box-and-whiskers plots of GFP intensity versus position along a neurite, normalized to GFP intensity at the soma, extracted from 2P images (setup 1) of neurites of (a) CoChR-GFP (n = 9 neurites taken from 4 cells from 3 slices from 3 mice) and (b) CoChR-KA2(1-150)-GFP expressing cells (soCoChR-GFP; n = 9 neurites taken from 6 cells from 2 slices from 2 mice) in acute slices. Red line denotes the median. Top and bottom edges of the box indicate the 75th and 25th percentiles, respectively. Top and bottom whiskers indicate the highest and lowest values, respectively. For CoChR the GFP fluorescence decays slower than the corresponding current (Fig. 3e); this is probably due to a contribution to the fluorescence from the dense distribution of out of focus processes from neighbouring cells.