Supplementary Figure 13: Mapping of functional connectivity in brain slices
From: Temporally precise single-cell-resolution optogenetics
(a) Currents recorded in whole-cell configuration from a CoChR-GFP expressing cell (same patched cell shown in Fig. 6b) while sequentially positioning the holographic spot on neighboring cells (λ = 920 nm, setup 2, photostimulation power: 120 µW/µm²; three light pulses at 50 Hz of 10 ms duration). Currents corresponding to neighboring cells numbered 3, 5 and 18 (green numbers) are shown in Fig. 6. The distances between each neighboring cell and the patched cell are reported next to each curve (grey numbers). Each trace is the average of three trials. Currents were recorded before the perfusion of synaptic receptor blockers (I TOT ) and after the perfusion of synaptic receptor blockers (I ART ). The current component sensitive to application of synaptic receptor blockers was: PSCs = I TOT - I ART . Black traces: I TOT where the ratio PSCs/I ART > 1. Gray traces: I TOT where the ratio PSCs/I ART < 1. Red traces: I TOT where no PSCs were observed (I TOT = I ART ). Blue traces: I ART . (b) Currents recorded in whole-cell configuration from a soCoChR-GFP expressing cell while sequentially positioning the holographic spot on neighboring cells (λ = 1030 nm, setup 1, photostimulation power: 120 µW/µm²; three light pulses at 50 Hz of 10 ms duration). Currents corresponding to neighboring cells numbered 6, 9 and 12 are shown in Fig. 6. The distances between each neighboring cell and the patched cell are reported next to each curve (grey numbers). Color code as in panel (a).
