Extended Data Fig. 2: Assessment of leaky expression from ConVERGD and INTRSECT constructs.
From: A single-vector intersectional AAV strategy for interrogating cellular diversity and brain function
a, Schematics for ConVERGD and INTRSECT constructs where no recombination has occurred, or upon Cre, Flp, or Cre and Flp-mediated recombination. Expected band sizes via PCR with specified primers are listed below. b, PCR of pAAV-hSyn-ConVERGD-eGFP-W3SL and pAAV-hSyn-INTRSECT-eYFP plasmids using the primer pairs described in a. c, Schematics for ConVERGD vectors where no recombination has occurred, or upon Cre, Flp, or Cre and Flp-mediated recombination. Schematics for vectors undergoing partial Flp-mediated recombination are also included. Expected band sizes via PCR with specified primers are listed below. d, Amplified PCR product from N2a cells transfected with hSyn-ConVERGD-eGFP-W3SL alone or with Cre, Flp, or Cre and Flp (pDIRE) expressing plasmids. mRNA extracted from these samples underwent a reverse transcriptase (RT) reaction to generate cDNA, or a no reverse transcriptase (no RT) reaction as a control. Each gel displays PCR products from template arising from the RT and no RT reactions. The gels are representative of results obtained across four independent sets of transfections. CV - ConVERGD; INTR – INTRSECT.
