Extended Data Fig. 6: ConVERGD shows specific, intersectional expression in the hippocampus of Calb1Cre; Slc17a7Flp mice.
From: A single-vector intersectional AAV strategy for interrogating cellular diversity and brain function
a, Representative images of labeled cells in the hippocampus upon injection of Cre-dependent eGFP AAV in Calb1Cre mice (left) or Flp-dependent mCherry AAV in Slc17a7Flp mice (right). b, Representative images of AAV-hSyn-ConVERGD-eGFP or AAV-hSyn-INTRSECT-Con/Fon-EYFP injected into the hippocampus of Calb1Cre, Slc17a7Flp, and Calb1Cre;Slc17a7Flp mice. Tissue sections in the top two rows reflect endogenous fluorescence while tissue sections in the bottom two rows were immunostained with GFP antibody. c, Representative images of AAV-hSyn-ConVERGD-ConFonvCon-eGFP injected into the hippocampus of Calb1Cre;Slc17a7Flp mice in the absence (left) or presence (right) of vCre-expressing AAV. Tissue sections were immunostained with GFP antibody. d, Representative images of AAV-hSyn-ConVERGD-ConFoff-eGFP injected into the hippocampus of Calb1Cre (left) or Calb1Cre;Slc17a7Flp (right) mice. Tissue sections were immunostained with GFP antibody. e, Representative images of AAV-hSyn-ConVERGD-CoffFon-eGFP injected into the hippocampus of Slc17a7Flp (left) or Calb1Cre;Slc17a7Flp (right) mice. Tissue sections were immunostained with GFP antibody. Images in a, c, d, and e are representative of results across 2 mice for each genotype; images in b are representative of results across 3 mice for each genotype. Scale bars are 100μm. WT - wild-type; Calb1 - calbindin 1; Slc17a7 - solute carrier family 17 member 7; INTR - INTRSECT; CV - ConVERGD.
