Extended Data Fig. 1: Comparison of ConVERGD-based constructs with varying promoter and posttranscriptional elements.
From: A single-vector intersectional AAV strategy for interrogating cellular diversity and brain function
a, FACS quantification of N2a cells co-transfected with an EYFP- or eGFP-expressing plasmid alone (Control, grey bars) or with recombinase plasmids expressing Cre (yellow bars), Flp (blue bars), or Cre and Flp (pDIRE, green bars). ConVERGD was tested in pAAV backbones containing different promoters and 3′ posttranscriptional regulatory elements. b, FACS quantification as in a but represented as percent of live, single cells that were counted positive for fluorescence. Bars represent the mean of all experiments. Error bars are SEM. CV - ConVERGD. Data points represent independent transfections with the following constructs: 56 (FLEx(FRT)eGFP alone or +pDIRE), 6 (CV-eGFP-W3SL +Flp, +pDIRE; CV-eGFP-WPRE, +Flp, +pDire; Ef1a-CV-eGFP-WPRE, +pDIRE) 5 (INTRSECT-EYFP, +Cre, +Flp, +pDIRE; CV-eGFP-W3SL, +Cre; CV-eGFP-WPRE+Cre; nEF-CV-eGFP-WPRE, +pDIRE) 4 (CV-EYFP, +Flp; CAG-CV-eGFP-W3SL, +Cre, +Flp, +pDIRE; CAG-CV-eGFP-WPRE; Ef1a-CV-eGFP-W3SL, +Cre, +Flp, +pDIRE; Ef1a-CV-eGFP-WPRE+Cre, +Flp; nEF-CV-eGFP-W3SL, +Cre, +Flp, +pDIRE; nEF-CV-eGFP-WPRE+Cre, +Flp) 3 (CV-EYFP+Cre, +pDIRE; CAG-CV-eGFP-WPRE+Cre, +Flp, +pDIRE).
