Extended Data Fig. 6: CAMs and microglia characterization after stroke in young and aged mice.
a) Representative flow cytometry dot-plots and gating strategy used for quantification of CAMs, microglia, macrophages and neutrophils 2 days after stroke in vehicle or clodronate-treated young and aged male mice brain. b-c) Flow cytometry quantification of CAMs, microglia, macrophages and neutrophils 2 days after stroke in vehicle or clodronate-treated young and aged mice brain. n=7 mice/group. d) Representative immunofluorescence images of CD206+ CAMs and Iba1+ microglia in perilesional area at 5 days after stroke in vehicle or CLO treated young and aged mice. Scale bar: 100 µm. e) Immunohistological quantification of CD206+ CAMs 1 day (left) and 5 days (right) after stroke. n=5 mice/group. f) Representative immunofluorescence images of Iba1+/CD68+ phagocytic microglia in perilesional area 1 day after stroke in vehicle or clodronate-treated young or aged mice. Scale bar: 100 µm. g) Immunohistological quantification of Iba1+ microglia 1 day and 5 days after stroke. n=5 mice/group. h) Immunohistological quantification of Iba1+/CD68+ phagocytic microglia 1 day and 5 days after stroke. n=5 mice/group. Multiple Mann-Whitney U-test with two-stage step up method for false discovery rate (b, c, e, g, h). Data are presented as mean values +/− SEM (b, c, e, g, h).
