Extended Data Fig. 2: Monitoring synaptic connections over time.
From: Transsynaptic labeling and transcriptional control of zebrafish neural circuits
a,a′, Dorsal view of mApple-CAAX-labeled neurons in the optic tectum (arrowheads) of a 6 dpf larva bearing Tg(isl2b.2:Gal4, myl7:tagRFP); Tg(UAS:GFP), Tg(QUAS:mApple-CAAX, he1.1:mCherry) and all trans-Tango components. Diffuse red fluorescent protein labeling (asterisk) is due to a secondary marker (my17:RFP) expressed in the heart. b,b′, In the same animal, labeling of the tectal neurons indicated in a (arrowheads) persisted at 2 weeks postfertilization (wpf; n = 10). Scale bars, 50 μm in a,a′ and 100 μm in b,b′. Images are representative of ten larvae from five independent experiments imaged at each time point.
