Extended Data Fig. 3: Social hierarchy and estrus cycle in negative self-experience modulation of emotion recognition.
(a, d) Experimental design of the EDT in (a) naïve and (d) self-experience condition. (b, e) Time spent sniffing demonstrators in stress (purple bars) or neutral (gray bars) state during the 6-min test, divided into three consecutive 2-min epochs, displayed by (left) dominant or (right) subordinate (b) naïve and (e) self-experience males (two-tailed multiple t-test, Bonferroni correction. N = 8 dominant and 13 subordinate observers). (c, f) Time spent sniffing demonstrators in stress (purple bars) or neutral (gray bars) state during the 6-min test, divided into three consecutive 2-min epochs, displayed by (left) estral or (right) diestral (c) naïve and (f) self-experienced females (two-tailed multiple t-test, Bonferroni correction. (N = 7 naïve estral and 7 diestral observers; N = 15 self-experience estral and 13 diestral observers). (g) Both dominant and subordinate self-experienced observers differ from naïve mice in the total social exploration towards stress, but not neutral (Three-way RM ANOVA, Bonferroni correction; N = 7 naïve and 7 self-experienced dominants, N = 11 naïve and 13 self-experienced subordinates). (h) Time spent grooming (two-tailed unpaired t-test; N = 7 dominant and N = 13 subordinate mice) and (i) corticosterone levels (two-tailed unpaired t-test; N = 5 dominant and N = 4 subordinate mice) by self-experience dominant (orange) and subordinate (dark green) observers. (j) Both estral and diestral negative self-experienced observers significantly differ from naïve mice in the total social exploration towards stress, but not neutral, demonstrators (Three-way RM ANOVA, Bonferroni correction; N = 5 naïve and 15 self-experienced estral, N = 7 naïve and 13 negative self-experienced diestral). (k) Estral (light purple) and diestral (purple) self-experienced female mice do not differ in the time spent grooming across the 6 min test (two-tailed unpaired t-test; N = 15 estral and N = 13 diestral observers). (l, m) Time in (l) seconds or (m) percentage of time spent sniffing demonstrators in stress (purple bars) or neutral (gray bars) state during the 6-min test displayed by (left) rank 2 or (right) rank 3 self-experienced subordinate mice (two-tailed multiple t-test, Bonferroni correction. N = 7 rank 2 and 6 rank 3 observers). (n) Time in seconds and (o) percentage of time spent sniffing demonstrators in stress (purple bars) or neutral (gray bars) state during the 6-min test, which occurred 24 hr after self-experience, displayed by observer mice that were in (left) estrus or (right) diestrus when self-experience of stress occurred (two-tailed multiple t-test, Bonferroni correction. (N = 16 in estrus and 6 in diestrus). Bar and line graphs show mean ± s.e.m. *P < 0.05. **P < 0.005. ***P < 0.0005.
