Extended Data Fig. 4: Silencing of CRF in mPFC modulates negative self-experience effect on stress emotion recognition.
(a, d) Experimental design of the EDT in (a) naïve and (d) negative self-experience condition. (b, c) Time spent sniffing demonstrators in stress (purple bars) or neutral (gray bars) state during the 6-min test, divided into three consecutive 2-min epochs, displayed by naïve (b) scrambled and (c) anti-CRF shRNA naïve observers (two-tailed multiple t-test, Bonferroni correction. (b) SCRAMBLED. 2 min: P = 0.0002, 4 min: P = 0.01, 6 min: P = 0.01, N = 14 observers. (c) anti-CRF shRNA. 2 min: P = 0.0006; 4 min: P = 0.03; 6 min: P = 0.01; N = 15 observers). (e, f) Time spent sniffing demonstrators in stress (purple bars) or neutral (gray bars) state during the 6-min test, divided into three consecutive 2-min epochs, displayed by negative self-experienced (e) scrambled and (f) anti-CRF shRNA observers (two-tailed multiple t-test, Bonferroni correction. (e) SCRAMBLED: no significant differences; N = 14 observers. (f) anti-CRF shRNA. 6 min: P = 0.01; N = 16 observers). (g, h) Time spent sniffing demonstrators in stress (purple bars) or neutral (gray bars) state during the 6-min test, divided into three consecutive 2-min epochs, displayed by anti-CRF shRNA negative self-experienced (g) dominants and (h) subordinates (two-tailed multiple t-test, Bonferroni correction. (g) DOMINANTS. 6 min: P = 0.02; N = 7 mice. (h) SUBORDINATES. 6 min: P = 0.052; N = 9 mice). Bar and line graphs show mean ± s.e.m. *P < 0.05; ***P < 0.0005.
