Extended Data Fig. 5: mPFC-CRF neurons activity during EDT and falcon restraint.
(a) CRF-Cre mice were injected into the mPFC with Syn.Flex.GCaMP6f.eYFP and implanted with a GRIN lens (n = 4/5mice). (b) Representative images of viral infection and GRIN lens placement of the five mice tested. (c, e) Experimental design of the EDT in (c) naïve and (e) negative self-experience condition. (d) Averaged percentage of time spent in the zone around a stress (purple bars) or a neutral (gray bars) demonstrator, displayed by naïve observers (two-tailed paired t-test; N = 4 observers). (f) Averaged percentage of time spent in the zone around a stress (purple bars) or a neutral (gray bars) demonstrator, displayed by negative self-experienced observers (left) with or (right) without preference towards stress demonstrators (two-tailed paired t-test. (left) Preference for stress: N = 3 mice. (right) No preference for stress: N = 2 mice. (g-j) Heatmaps of entrances in stress (g, i) or neutral (h, j) zone associated with neuronal inactivation in (g, h) naïve and (i, j) negative self-experience condition. (k) Experimental design: mice were placed for 10 min inside an empty cage (before restraint stress), and subsequently moved inside a 50 ml falcon for 30 min. (l, m) Average peaks’ (l) frequency and (m) amplitude before (yellow) and after (purple) restrain stress (two-tailed Wilcoxon matched-pairs signed ranks test. (l) Number of pairs: 62, p < 0.0001. (m) Number of pairs: 62, p = 0.84). Bar and line graphs show mean ± s.e.m. ***P < 0.0005.
