Extended Data Fig. 2: Effects of different stressful experiences on emotion recognition.
(a, c) Experimental design of the EDT with one footshock (green) vs one neutral (gray) demonstrator in (a) naïve and (c) footshock self-experienced observers. (b, d) Time (in seconds) spent sniffing demonstrators in footshock stress (green bars) or neutral (gray bars) state during the 6-min test, divided into three consecutive 2-min epochs, displayed by (b) naïve and (d) footshock self-experienced observer mice (two-tailed multiple t-test, Bonferroni correction. N = 8 naïve and 16 footshock self-experience observers). (e) Mice in naïve (white) and footshock self-experience (blue) condition do not differ based on the amount of time spent grooming across the 6 min test (two-tailed unpaired t-test; N = 8 naïve and N = 16 footshock self-experienced mice). (f) No significant correlation was found between discrimination index (in y axis) and grooming (in x axis) of footshock self-experienced observer (two-tailed Pearson correlation; N = 16 mice). (g, i) Experimental design of the EDT with one restraint stress (purple) vs one neutral (gray) demonstrator in (g) naïve and (i) footshock-experienced mice. (h, j) Time spent sniffing demonstrators in restraint stress (purple bars) or neutral (gray bars) state during the 6-min test, divided into three consecutive 2-min epochs, displayed by (h) naïve and (j) footshock-experienced observer mice (two-tailed multiple t-test, Bonferroni correction. N = 14 naïve and 11 footshock-experience observers). (k) Naïve (white) and footshock-experienced (green) observers did not differ based on the amount of time spent grooming across the 6 min test (two-tailed unpaired t-test; N = 25 naïve and N = 11 footshock-experienced mice). (l) No significant correlation was found between discrimination index (in y axis) and grooming (in x axis) of footshock-experienced observer (two-tailed Pearson correlation; N = 11 mice). (m, o) Experimental design of the EDT with one fear vs one neutral demonstrator in (m) naïve and (o) restraint-experienced mice. (n, p) Time spent sniffing demonstrators in fear (red bars) or neutral (gray bars) state during the 6-min test, divided into three consecutive 2-min epochs, displayed by (n) naïve and (p) restraint-experienced observer mice (two-tailed multiple t-test, Bonferroni correction. N = 10 naïve and 12 restraint-experience observers). (q) Naïve (white) and restraint-experienced observers (purple) did not differ based on the amount of time spent grooming across the 6 min test (two-tailed unpaired t-test; N = 10 naïve and N = 12 restraint-experienced mice). (r) No significant correlation was found between discrimination index (in y axis) and grooming (in x axis) of footshock-experienced observer (two-tailed Pearson correlation; N = 12 mice). Bar and line graphs show mean ± s.e.m. *P < 0.05, ***P < 0.0005.
