Extended Data Fig. 1: Region-specific photoinactivation of ALM and tjM1.
From: Separating cognitive and motor processes in the behaving mouse
a. Percentage of time spent moving (see Methods) in a session determined by video recordings using the side view, bottom view, and both views. Points indicate each of the 12 randomly selected sessions used for this analysis. Error bars denote standard deviation across sessions (n = 12). b. Effect of delay epoch photoinactivation on behavioral performance (middle column) and uninstructed movements (right column) when photoinactivation was directed to the MC (ALM + tjM1; top row; same data as Fig. 1g–in = 14 sessions, 2 mice), the ALM (middle row, n = 15 sessions, 2 mice), and the tjM1 (bottom, n = 9 sessions, 2 mice). Photoinactivation of ALM and tjM1 led to similar behavioral impairment and reduction in uninstructed movements, with larger effects observed with MC (ALM + tjM1) photoinactivation. c. Tongue length during control and go cue/water drop photoinactivation trials for delayed-response (left) and water-cued (right) contexts. Blue traces indicate right lickport contacts, red traces indicate left contacts, and black traces indicate no contact. Vertical dashed line indicates go cue or water drop onset. Blue shaded region indicates photoinactivation period. d. Percentage of time with tongue visible during photoinactivation period for DR trials (left) and WC trials (right). Each colored point indicates mean value for an animal (n = 4 animals), individual animals are connected by black lines. Light gray lines denote individual sessions (n = 10 sessions). Bars are the mean across all sessions. Asterisks denote significant differences (p < 0.05) between control and photoinactivation trials (Percent reduction on all DR trials: 19 ± 7%, mean ± s.d., p = 1.6e-05; DR left trials: 20 ± 8%, p = 3.0e-05; DR right trials: 20 ± 7%, p = 1.6e-05; All WC trials: 4 ± 8%, p = 0.154; WC left trials: 1% ± 9%, p = 0.702; WC right trials: 7% ± 5%, p = 0.002; paired two-sided t-test, n = 10 sessions). Error bars indicate standard deviation across sessions. In WC trials, tongue protrusion was only significantly impaired on one trial type, while ability to successfully contact the lickport was impaired in all conditions (see Fig. 1c).
