Supplementary Figure 6: TR region changes at specific gene sets and human variation in SUMO-modifying genes.
From: Influenza virus infection causes global RNAPII termination defects
a, TR plots at termination region of active (RPKM > 1 in 50% of samples) multi-exonic (upper panels) and mono-exonic (lower panels) genes in uninfected A549 cells and 6 or 12 hpi with NS1 or NS1-SUMO virus. Data is shown for two replicates in each condition. b, Scatter plot showing the relationship between the log fold-change (logFC) in gene expression between NS1-SUMO virus and NS1 virus-infected A549 cells at 6 hpi, and the logFC in transcript levels in 5,000 bp 3’ gene-flanking regions. Genes with a significant increase or decrease in expression in NS1-SUMO vs NS1 infected A549 cells at 6 hpi are highlighted in red and green, respectively. All other genes are highlighted in grey. Solid and dotted lines correspond to the regression line and 95% confidence interval for genes with significant expression differences, with the R2 and significance shown at the top-left. c, Analysis of SUMO-modifying gene mutations using a compendium of protein-coding genetic variation in 60,706 humans from the ENCODE Consortium. The probability of being loss-of-function intolerant (intolerant of both heterozygous and homozygous of variants) is shown for all genes compared to SUMO-modifying genes.
