Supplementary Figure 2: Control experiments.
From: Influenza virus infection causes global RNAPII termination defects
Control experiments for Fig. 2. a, IF of ectopically expressed GFP, NS1, NS1-KR and NS1-SUMO in A549 (scale bar indicates 100um). b, Pull-down assay between recombinant NS1, NS1-KR, NS1-SUMO and PSD95. PBS served as a negative control. Coomassie staining SDS-PAGE showing purified recombinant proteins (left) and eluted complexes (right). c-e, Structural modeling of SUMOylated NS1 tail and the indicated PDZ domain containing-proteins GIPC2 (c), DVL1 (d) and PSD95 (e). f, Schematic diagram of plaque selection and analysis. All plaques selected after 24 hpi with NS1-SUMO virus indicate that the virus has reverted back to NS1 virus (WT virotype) by mutagenesis of its C-term SUMO extension. This result indicates that NS1-SUMO virus can infect but it is compromised in its ability to bud and spread from cell-to-cell.
