Extended Data Fig. 8: Relative orientation of RF3 with GDPCP or GDPNP in previous structures versus that in structure II-B.
From: The ribosome termination complex remodels release factor RF3 and ejects GDP
The structures are aligned based on the 23S rRNA. a, The conformation of RF3-GDPCP in structure II-B on the rotated ribosome (colored by domain) is highly similar to that of RF3-GDPCP reported in a previous study with the antimicrobial peptide apidaecin (state IV; rotated ribosome) (PDB 6GXO, gray)3. b, Same as in panel (a) relative to previous crystal structures of RF3-GDPNP bound to the rotated E. coli 70S ribosome (PDB 4V85)6 or RF3-GDPCP bound to the rotated Thermus thermophilus 70S ribosome (PDB 4V8O)7. c-f, Close-up views of the sarcin-ricin loop (SRL) and domain I of RF3 from (a) and (b). The catalytic histidine 92 in switch 2 (sw2) is located 9 to 13 Å away from the non-bridging oxygen of A2662 in the SRL, and RF3 is therefore in a GTP-inactive state on the ribosome. Switch 1 (sw1) is orange.
