Extended Data Fig. 7: 2-aminopurine (2AP) lifetime analyses of human and mouse menRNA.
From: Structural basis of NEAT1 lncRNA maturation and menRNA instability
a-d, Time-resolved 2AP fluorescence decay time traces of mouse menRNA (a), the acceptor stem of the human menRNA (b) and its U57C/U58C (c) and G1A/G2A (d) mutants. IRF: instrument response function. e, Deconvoluted individual lifetimes (indicated in ns) and relative amplitudes derived from (a-d), also tabulated in Supplementary Table 1. The amplitudes for the short, intermediate, and long lifetimes are shown in violet, green, and yellow, respectively, as stacked columns. Norm.: Normalized. The amplitude-weighted averaged lifetimes τavg are summarized in Fig. 2f. Data collected on the PTI EasyLife spectrofluorometer.
