Fig. 2: CerS6 contains an acyl chain-binding tunnel buried deep in the membrane.
From: Structural basis of the mechanism and inhibition of a human ceramide synthase
a, Cutaway molecular surface representation of the CerS6 TM region, revealing the presence of a long central cavity spanning the entire length of the protein. The palmitoyl chain covalently attached to His211 is bound in a narrow tunnel on the ER luminal half of the central cavity. b, Coulombic potential map in the region around the covalent linkage of the acyl chain to His211. c–e, CerS6’s acyl-binding tunnel, viewed from the membrane plane (c and d) or the ER face (e). Side chains lining and capping the tunnel are shown as sticks. Hydrogen bonds in the active site are shown as dashed lines.
