Extended Data Fig. 2: Cryo-EM analysis of Pol II-Alu-LA.
From: Mechanism of mammalian transcriptional repression by noncoding RNA
a, Cryo-EM micrograph representative field of view, -1.9 μm defocus. One of a total of 16,470 micrographs is shown. b, Representative 2D classes. c, Processing tree outlining the steps taken to generate the Pol II-Alu-LA reconstruction. Classes with well-resolved clamp and stalk domains are indicated. d, Angular distribution plot for the Pol II-Alu-LA reconstruction. e, Fourier shell correlation plot for the Pol II-Alu-LA reconstruction and the Pol II-Alu-LA phase-randomized masked maps. f, Locally-filtered, non-B-factor-sharpened Pol II-Alu-LA reconstruction colored by estimated local resolution.
