Extended Data Fig. 6: CUT&RUN tracks at candidate X-linked gene locus. | Nature Structural & Molecular Biology

Extended Data Fig. 6: CUT&RUN tracks at candidate X-linked gene locus.

From: Temporal and regional X-linked gene reactivation in the mouse germline reveals site-specific retention of epigenetic silencing

Extended Data Fig. 6

(A) Integrative Genomics Viewer tracks for total H3K27me3 normalized reads (dark blue) and allele-specific reads (light blue: Xi/CAST; red: Xa/B6) at the Gjb1 (silent gene), Zmym3 (very-late reactivated gene) and Nono (early-reactivated gene) locus. Each replicate is shown at E11.5 and E12.5. (B-D) Integrative Genomics Viewer tracks for total H3K27me3 normalized reads (dark blue) and allele-specific reads (light blue: Xi/CAST; red: Xa/B6) at (B) the Med14 early reactivated gene, (C) the Kif4 early-reactivated gene, and (D) the Cacna1f silent gene in Count Per Million mapped reads (CPM, bin 10). Orange blocks are statistically-called H3K27me3 broad domains by MACS2, compared to IgG. Location is given in mm10 genome, with gene isoforms extracted from Integrative Genome viewer and UCSC.

Back to article page