Extended Data Fig. 1: Differential gene expression upon female PGC development and key markers of soma and PGC.
(A) PCA of the 30 most differentially expressed genes (DEGs) that contributed to lineage segregation. The different stages are denoted by different colours. Orange arrows represent genes contributing to PGC lineage, and blue arrows represent genes contributing to soma lineages. (B-D) Volcano plots represent differentially expressed genes (DEGs) between the two developmental stages of female PGCs. Differential expression was measured by TMM method (trimmed mean of M values, see Method section), followed by Benjamini–Hochberg correction. Genes with an adjusted P value < 0.05 were considered significant. A few transcriptional changes have been observed in migratory PGCs. Changes arise once PGCs colonize the gonads. Some examples of DEGs are highlighted. Red dots represent upregulated genes, and blue dots represent downregulated genes. X-linked genes are shown in orange.
