Extended Data Fig. 4: ELL3 is localized at the meiotic spindle.
From: Maternal ELL3 loss-of-function leads to oocyte aneuploidy and early miscarriage
a, Representative images of ELL3-RFP localization in mouse oocytes at GV, GVBD, MI, and MII stages. Red, RFP; green, microtubules; blue, DNA. Scale bar, 20 μm. b, Western blot showing the specificity of the ELL3 polyclonal antibody against the C-terminal region of ELL3 (pAb-CT) using WT, ELL3 KO and ELL3 KO HCT 116 cells with GFP-ELL3 expression. Red asterisks pointing to the distinct bands corresponding to ELL3 or GFP-ELL3. c, Mouse oocytes were immunostained with the validated ELL3 pAb-CT. Green, ELL3; blue, DNA. Scale bar, 20 μm. d, Immunostaining showing Ell3 KD efficiency in mouse oocytes. Scale bar, 20 μm. e, ELL3 intensity in control (n = 6) and Ell3 KD (n = 6) mouse oocytes. f, Representative images of meiotic resumption in control and Ell3 KD mouse oocytes at GVBD stage. Scale bar, 100 μm. g, GVBD rate of control (n = 92) and Ell3 KD (n = 145) mouse oocytes. Data are presented as mean ± s.e.m. (e,g). Data are from more than three independent experiments (e,g). N indicated the cumulative number of independent oocytes and independent experiments are denoted by dots (g). Statistical analysis was performed using a two-tailed, unpaired Student’s t-test (e,g). ns, not significant.
