Extended Data Fig. 10: ELL3 and TPX2 safeguard proper spindle assembly through balancing KIF11.
From: Maternal ELL3 loss-of-function leads to oocyte aneuploidy and early miscarriage
a, Time-lapse 3D light-sheet microscopy imaging of control and Kif11 KD mouse oocytes expressing H2B-mCherry. Scale bar, 20 μm. b, Left: Path analysis after Kif11 KD, showing the distance to the equator plane across MI. Each colored curve matches one chromosome. Right: Speed of chromosome movement in Kif11 KD mouse oocyte. c, Time-lapse 3D light-sheet microscopy imaging of DMSO and monastrol treated mouse oocytes expressing H2B-mCherry. Scale bar, 20 μm. d, Left: Path analysis of the monastrol treated mouse oocyte, showing the distance to the equator plane across MI. Each colored curve matches one chromosome. Right: Speed of chromosome movement in monastrol treated oocyte. e, Western blot showing TPX2 expression in control (n = 150), Tpx2 KD (n = 150) and Tpx2 KD + Tpx2ΔKID (n = 150) mouse oocytes. f, Representative images of 3D reconstructed spindle in control, Tpx2 KD + Tpx2ΔKID and Tpx2 KD + Tpx2ΔKID + Ell3 KD mouse oocytes at different stages. Oocytes at about 2 h after GVBD (early), about 4 h after GVBD (middle) and about 6 h after GVBD (late) are shown. Scale bar, 20 μm. g, Distance to the equator plane across MI. Each colored curve matches one chromosome. Data are presented as mean with shade represents 95 confidence interval (b,d).
