Extended Data Fig. 4: G392R mutant is anion permeable.
From: Structure and function of a broad-range thermal receptor in myriapods
a, Voltage ramps (800 ms) ranging from −80 to +80 mV were used to record current in different extracellular solutions of G392R mutant transfected cells. Extracellular solution (140 mM NaCl, 5 mM MES, 5 mM EDTA, pH 3.5) was substituted with sucrose-supplemented extracellular solution (14 mM NaCl, 5 mM MES, 5 mM EDTA, pH 3.5, osmolarity constant is adjust by sucrose), respectively in a. b, The reversal potentials tested in hPAC-KO cells expressing G392R (n = 9 biological replicates). Data points and bars represent mean ± sem.
