Extended Data Fig. 7: Cation selectivity pattern of I400A and C401A.
From: Structure and function of a broad-range thermal receptor in myriapods
a and c, Voltage ramps (500 ms) ranging from −60 to +60 mV were used to record current in different extracellular solutions of I400A or C401A transfected cells, respectively. Bath solution (140 mM NaCl, 5 mM MES, pH 3.5) was substituted with different cationic compositions (140 mM KCl, 140 mM CsCl, 140 mM LiCl, 110 mM CaCl2, 110 mM MgCl2, or 110 mM BaCl2). b, Cation selectivity in I400A transfected cells (n = 6 biological replicates; Mg2+, P = 0.3487; Ba2+, P = 0.6575; Ca2+, P = 0.6855; Cs+, P = 0.9; Li+, P = 0.0001; K+, P = 0.0002). d, Cation selectivity in C401A transfected cells (n = 9 biological replicates; Mg2+, P = 0.8969; Ba2+, P = 0.6443; Ca2+, P = 0.1272; Cs+, P = 0.0992; Li+, P = 0.0015; K+, P = 0.114). Data points and bars represent mean ± sem (two-tailed t-test. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; N.S, not statistically significant).
