Extended Data Fig. 4: crRNA recognition.
From: Structural insights into RNA-guided RNA editing by the Cas13b–ADAR2 complex
(a–d) Recognition of the crRNA (a), U4 and C5 (b), the possible seed sequence (c), and the 3ʹ nucleotide (d). Hydrogen bonds are depicted with green dashed lines. (e) SDS–PAGE analysis of the purified PspCas13b and its mutants. The proteins were visualized with CBB. All experiments were performed at least three times. (f) In vitro pre-crRNA processing experiments. The pre-crRNA was incubated with the purified WT PspCas13b or its mutants at 37 °C for 30 min, and then analyzed by denaturing urea–PAGE. The nucleic acids were visualized with SYBR Gold. All experiments were performed at least three times.
